Comparative Study of Isoflavone Synthesis Genes in Two Wild Soybean Varieties Using Transcriptomic Analysis

Soybean is an important food crop that contains high amounts of isoflavones. However, due to the expression multiple genes, different soybean seeds have isoflavone compositions. The underlying mechanisms for this complexity remain unknown. In study, we identified potential differentially expressed genes (DEGs) in two wild cultivars, ZYD7068 (high isoflavone) and ZYD7194 (low isoflavone), at seed developmental stages using RNA-seq technology compared their differences content. A total 1067 6479 metabolized were R6 R8 stages, respectively. Subsequent analysis KEGG pathway revealed three these differential involved Isoflavonoid biosynthesis Flavone flavonol stage. 80 TF encoding MYB, bZIP, WRKY A1 vs. B1 A3 B3. Eight duplicates both showed same trend stages. To confirm results RNA-seq, qRT-PCR was performed analyze six (DEGs). consistent with RNA-seq. We found four (Glyma.13G173300, Glyma.13G173600, Glyma.14G103100, Glyma.17G158900) may be positive regulation synthesis, while (Glyma.04G036700 Glyma.19G030500) negative synthesis. These findings suggest observed difference levels between cultivars attributable later development.